The two most important parameters in objective design are the numerical aperture (which determines the fine detail in the image) and the magnification, which decreases the area that is imaged. For ...

The beam is then steered via an optical path to fill the back aperture of an objective lens (numerical aperture of 1.4) housed in an inverted microscope. The laser power and position of the beam are ...

The size of the Airy disk depends on the wavelength of the light (λ) and the numerical aperture (NA) of the objective lens. The spatial resolution of the microscope is determined by the ability to ...